Retinal Homeobox Protein Rx (RAX) (internal) Antikörper

Antigen: Retinal Homeobox Protein Rx (RAX)

Synonyme: RX, MCOP3, Rx, ey1, ey-1, 7530406A22Rik, E130303K03Rik

Epitope: internal

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350791

Produktbeschreibung

Weitere Bezeichnung: RAX (internal region)

Swiss-Prot: Q9Y2V3

Immunogen: A synthetic peptide from the internal region of human RAX conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Beschreibung: Function: Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation. Binds to the photoreceptor conserved element-I (PCE-1/Ret 1) in the photoreceptor cell-specific arrestin promoter. Defects in RAX are the cause of microphthalmia isolated type 3 (MCOP3). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataractand other abnormalities like cataract may also be present. Tissue specificity: Expressed in the developing eye and weakly expressed in the adult retina. Subcellular location: Nucleus Also known as: Retina and anterior neural fold homeobox protein, Retinal homeobox protein Rx.

Spezifität: Appears to be specific for RAX.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Furukawa, Kozak, Cepko: "rax, a novel paired-type homeobox gene, shows expression in the anterior neural fold and developing retina." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 7, pp. 3088-93, 1997 (PubMed).

Kimura, Singh, Wawrousek et al.: "Both PCE-1/RX and OTX/CRX interactions are necessary for photoreceptor-specific gene expression." in: The Journal of biological chemistry, Vol. 275, Issue 2, pp. 1152-60, 2000 (PubMed).

Voronina, Kozhemyakina, OKernick et al.: "Mutations in the human RAX homeobox gene in a patient with anophthalmia and sclerocornea." in: Human molecular genetics, Vol. 13, Issue 3, pp. 315-22, 2004 (PubMed).

Rohde, Klein, Møller et al.: "Rax : developmental and daily expression patterns in the rat pineal gland and retina." in: Journal of neurochemistry, 2011 (PubMed). Weitere Details: The nitrocellulose membrane was blocked with 2% (w/v) skim milk in phosphate-buffered saline (PBS) and incubated overnight at 4