Proliferating Cell Nuclear Antigen (PCNA) (C-Term) Antikörper

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyme: MGC8367, PCNAR, Pcna/cyclin, pcna

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350772

Produktbeschreibung

Weitere Bezeichnung: Prolifeing cell nuclear antigen (PCNA) (c-terminal region)

Swiss-Prot: P04961

Immunogen: A synthetic peptide from the c-terminal region of rat Proliferating cell nuclear antigen (PCNA) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human and rat.

Format: Lyophilized

Isotyp: IgG

Beschreibung: Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involvement in disease: Antibodies are present in sera from patients with systemic lupus erythematosus. Also known as: Cyclin, DNA polymerase delta auxiliary protein, Polymerase delta accessory protein, Proliferating Cell Nuclear Antigen.

Spezifität: Appears to be specific for PCNA.

Anwendungen

Applikationshinweise: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Reinheit: IgG

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Almendral, Huebsch, Blundell et al.: "Cloning and sequence of the human nuclear protein cyclin: homology with DNA-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1575-9, 1987 (PubMed).

Deloukas, Matthews, Ashurst et al.: "The DNA sequence and comparative analysis of human chromosome 20." in: Nature, Vol. 414, Issue 6866, pp. 865-71, 2002 (PubMed).

Sakurai, Kitano, Yamaguchi et al.: "Structural basis for recruitment of human flap endonuclease 1 to PCNA." in: The EMBO journal, Vol. 24, Issue 4, pp. 683-93, 2005 (PubMed).

Unk, Hajduu, Fuatyol et al.: "Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 48, pp. 18107-12, 2006 (PubMed).