Proteinase 3 (PRTN3) (C-Term) Antikörper

Antigen: Proteinase 3 (PRTN3)

Synonyme: MBN, MBT, NP4, P29, PR3, ACPA, AGP7, PR-3, CANCA, C-ANCA, mPR3, ela2, MGC132250

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB), Immunhistochemie (IHC), Durchflusszytometrie (FACS)

Produktnummer: ABIN350763

Produktbeschreibung

Weitere Bezeichnung: Pr3 (Wegener autoantigen) (c-terminal region)

Swiss-Prot: P24158

Immunogen: A synthetic peptide from the c-terminal region of human Pr3 (Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Beschreibung: FUNCTION: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to s. Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.<||a href="javascript:void(0);/*1257874674096*/"><||/a> Also known as: Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen, PR-3, P29, C-ANCA antigen, Neutrophil proteinase 4, MBN, PRTN3, NP-4.

Spezifität: Appears to be specific for Pr3.

Anwendungen

Applikationshinweise: WB, IHC, flow cytometry. A dilution of 1: 200 to 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

WB on human PMN (peripheral blood mononuclear cells isolated from buffycoat. Denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (ABIN350763) at 1: 500 dilution. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (ABIN350763) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (ABIN350763).

Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (ABIN350763) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (ABIN350763).

Publikationen

Publikationen: Labbaye, Musette, Cayre: "Wegener autoantigen and myeloblastin are encoded by a single mRNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 20, pp. 9253-6, 1991 (PubMed).

Jenne, Tschopp, Luedemann et al.: "Wegener's autoantigen decoded." in: Nature, Vol. 346, Issue 6284, pp. 520, 1990 (PubMed).

Duke-Cohan, Morimoto, Rocker et al.: "A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)." in: The Journal of biological chemistry, Vol. 270, Issue 23, pp. 14107-14, 1995 (PubMed).

Fujinaga, Chernaia, Halenbeck et al.: "The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies." in: Journal of molecular biology, Vol. 261, Issue 2, pp. 267-78, 1996 (PubMed).

Grimwood, Gordon, Olsen et al.: "The DNA sequence and biology of human chromosome 19." in: Nature, Vol. 428, Issue 6982, pp. 529-35, 2004 (PubMed).