Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2/4 (HCN2/4) (1t cytoplasmic domain) Antikörper

Antigen: Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 2/4 (HCN2/4)

Synonyme: HCN4, hcn4, BCNG2, HAC-1, BCNG-2, HCN2, wu:fj61d07, si:dkey-28h18.1

Epitope: 1t cytoplasmic domain

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350762

Produktbeschreibung

Weitere Bezeichnung: potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 and 4 (HCN2, HCN4) (1t cytoplasmic domain)

Swiss-Prot: Q9UL51

Immunogen: A synthetic peptide from the 1t cytoplasmic domain of human potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 and 4 (HCN2, HCN4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Beschreibung: HCN2: FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in neonatal and adult ventricle and in brain. Highly expressed in the pyramidal layer in hippocampus, in anterior dorsal nucleus in thalamus, in the mammillary nucleus in hypothalamus, in red nucleus, in trigeminal mesencephalic, spinal and principal nuclei, in cochlear and trapezoid nuclei and in the dorsal tegemental nucleus. INDUCTION: By thyroid hormones in hypothyroid animals. DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. HCN4: FUNCTION: Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP. May mediate responses to sour stimuli. SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in thalamus, testis and in heart, both in ventricle and atrium. Detected at much lower levels in amygdala, substantia nigra, cerebellum and hippocampus. Also known as: HCN2 (Brain cyclic nucleotide-gated channel 2, BCNG-2, BCNG2, HAC-1), HCN4.

Spezifität: Appears to be specific for HCN2, HCN4.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ludwig, Zong, Stieber et al.: "Two pacemaker channels from human heart with profoundly different activation kinetics." in: The EMBO journal, Vol. 18, Issue 9, pp. 2323-9, 1999 (PubMed).

Shi, Wymore, Yu et al.: "Distribution and prevalence of hyperpolarization-activated cation channel (HCN) mRNA expression in cardiac tissues." in: Circulation research, Vol. 85, Issue 1, pp. e1-6, 1999 (PubMed).

Pachucki, Burmeister, Larsen: "Thyroid hormone regulates hyperpolarization-activated cyclic nucleotide-gated channel (HCN2) mRNA in the rat heart." in: Circulation research, Vol. 85, Issue 6, pp. 498-503, 1999 (PubMed).

Monteggia, Eisch, Tang et al.: "Cloning and localization of the hyperpolarization-activated cyclic nucleotide-gated channel family in rat brain." in: Brain research. Molecular brain research, Vol. 81, Issue 1-2, pp. 129-39, 2000 (PubMed).

Ueda, Nakamura, Hayashi et al.: "Functional characterization of a trafficking-defective HCN4 mutation, D553N, associated with cardiac arrhythmia." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27194-8, 2004 (PubMed).

Kimura, Kitano, Nakajima et al.: "Hyperpolarization-activated, cyclic nucleotide-gated HCN2 cation channel forms a protein assembly with multiple neuronal scaffold proteins in distinct modes of protein-protein interaction." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 9, Issue 7, pp. 631-40, 2004 (PubMed).