Pan rho (A, B, C, F, Q), RAC (1, 2, 3) and CDC42 Antikörper

Antigen: Pan rho (A, B, C, F, Q), RAC (1, 2, 3) and CDC42

Synonyme: PIXA, COOL2, MRX46, Cool-2, KIAA0006, alphaPIX, alpha-PIX, 1600028C08Rik, 1700038J06Rik, 4930592P22Rik, ARHGEF6

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350720

Produktbeschreibung

Immunogen: A synthetic peptide as a part of human/rat/mouse/chicken/dog/frog (and more species) Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Beschreibung: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Also known as: Rho-related GTP-binding protein RhOA, RhOB, RhOC, RhOF, RhOQ, RAC1, RAC2, RAC3, CDC42.

Spezifität: Appears to be specific for RhO family.

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

WB on rat brain lysate using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum (ABIN350720) at 1 : 1000 dilution.

IHC on rat midbrain using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum (ABIN350720) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum (ABIN350720) at 1: 2000 dilution. DAPI counter stained.

Publikationen

Publikationen: Munemitsu, Innis, Clark et al.: "Molecular cloning and expression of a G25K cDNA, the human homolog of the yeast cell cycle gene CDC42." in: Molecular and cellular biology, Vol. 10, Issue 11, pp. 5977-82, 1990 (PubMed).

Shinjo, Koland, Hart et al.: "Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 24, pp. 9853-7, 1991 (PubMed).

Nakamura, Asano, Shindo-Okada et al.: "Cloning of the RhoB gene from the mouse genome and characterization of its promoter region." in: Biochemical and biophysical research communications, Vol. 226, Issue 3, pp. 688-94, 1996 (PubMed).

Westmark, Bartleson, Malter: "RhoB mRNA is stabilized by HuR after UV light." in: Oncogene, Vol. 24, Issue 3, pp. 502-11, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).