Nerve Growth Factor Receptor (NGFR) (cytoplasmic domain) Antikörper

Antigen: Nerve Growth Factor Receptor (NGFR)

Synonyme: CD271, p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, MGC148470, NGFR, wu:fb38c02, wu:fc10h01, p75, LNGFR, RNNGFRR, nrh1, NRH1b, p75ntr, tnfrsf16, MGC131034

Epitope: cytoplasmic domain

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350712

Produktbeschreibung

Weitere Bezeichnung: p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR) (extracellular domain)

Swiss-Prot: P08138

Immunogen: A synthetic peptide from the extracellular domain of human p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including mouse and rat.

Format: Lyophilized

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/<||a href="http://www.osenses.com/bex-family-c-21_106.html" >BEX3<||/a>. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with <||a href="http://www.osenses.com/advanced_search_result.php?antigen=sqstm1&x=0&y=0" >SQSTM1<||/a> which bridges NGFR to NTRK1. Interacts with <||a href="http://www.osenses.com/bex-family-c-21_106.html" >BEX1<||/a>. Interacts with ARMS and NTRK1. Can form a ternary complex with NTRK1 and ARMS and this complex is affected by the expression levels of ARMS. An increase in ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts with LINGO1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. Also known as: Low-affinity nerve growth factor receptor, NGF receptor, Gp80-LNGFR, p75 ICD, Low affinity neurotrophin receptor p75NTR, p75, LNGFR, p75NTR, RNNGFRR.

Spezifität: Appears to be specific for p75NTR.

Anwendungen

Applikationshinweise: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Reinheit: IgG

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

WB on rat trigeminal lysate (10% gel, 1:29. Reduced, denatured) using Rabbit antibody to extracellular domain of p75NTR (low affinity NGF receptor, p75, LNGFR, p75NTR): IgG (ABIN350712) at a concentration of 30 µg/ml. blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (0.1 x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature. One single band of about 45 Kda will be detected. Pre-absorption with the immunising peptide abolished the detected band completely.

Publikationen

Publikationen: Johnson, Lanahan, Buck et al.: "Expression and structure of the human NGF receptor." in: Cell, Vol. 47, Issue 4, pp. 545-54, 1986 (PubMed).

Chapman, Eckart, Kaufman et al.: "O-linked oligosaccharide on the 75-kDa neurotrophin receptor." in: Journal of neurochemistry, Vol. 66, Issue 4, pp. 1707-16, 1996 (PubMed).

Khursigara, Orlinick, Chao: "Association of the p75 neurotrophin receptor with TRAF6." in: The Journal of biological chemistry, Vol. 274, Issue 5, pp. 2597-600, 1999 (PubMed).

Ye, Mehlen, Rabizadeh et al.: "TRAF family proteins interact with the common neurotrophin receptor and modulate apoptosis induction." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30202-8, 1999 (PubMed).

Irie, Hachiya, Rabizadeh et al.: "Functional interaction of Fas-associated phosphatase-1 (FAP-1) with p75(NTR) and their effect on NF-kappaB activation." in: FEBS letters, Vol. 460, Issue 2, pp. 191-8, 1999 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Mi, Lee, Shao et al.: "LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex." in: Nature neuroscience, Vol. 7, Issue 3, pp. 221-8, 2004 (PubMed).