Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C) Antikörper

Antigen: Microtubule-Associated Protein 1 Light Chain 3 gamma (MAP1LC3C)

Synonyme: LC3C, MAP1LC3C, MGC154878

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350468

Produktbeschreibung

Weitere Bezeichnung: microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C, MAP1A/MAP1B LC3 C)

Swiss-Prot: Q9BXW4

Immunogen: A synthetic peptide as a part of human microtubule-associated protein 1 light chain 3 gamma (MAP1LC3C, MAP1A/MAP1B LC3 C) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Beschreibung: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes). SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note: LC3-II binds to the autophagic membranes. TISSUE SPECIFICITY: Most abundant in placenta, lung and ovary. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Also known as: Microtubule-associated proteins 1A/1B light chain 3C, MAP1LC3C, MAP1A/MAP1B LC3 C, MAP1A/1B light chain 3 C, MAP1 light chain 3-like protein 3,autophagy-related protein LC3 C,autophagy-related ubiquitin-like modifier LC3C.

Spezifität: Appears to be specific for MAP1LC3C.

Anwendungen

Applikationshinweise: IHC, WB. Use at a dilution of 1 : 200 to 1 : 5000. The optimal dilution should be determined by the end user.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Forschungsgebiet: Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: He, Dang, Dai et al.: "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 29278-87, 2003 (PubMed).

Tanida, Sou, Ezaki et al.: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).