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Tre1 (AA 320-370) Antikörper

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Antigen
Epitop
AA 320-370
Reaktivität
Fruchtfliege (Drosophila melanogaster)
Wirt
Kaninchen
Klonalität
Polyklonal
Applikation
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from aa region 320-370 of Drosophila Tre1 conjugated to blue carrier protein was used as the antigen.
Spezifität Specific for Tre1.
Reinigung Whole serum
Hintergrund Function: Taste receptor sensitive to trehalose.
Subcellular location: Cell membrane, Multi-pass membrane protein
Tissue specificity: Expressed ubiquitously in adult tissues. Highest expression levels are seen in taste sensory cells of the labellar chemosensory neurons and tarsi.
Developmental stage: Expressed throughout embryo to adult stages. Also known as: Trehalose receptor 1, Tre1.
Applikationshinweise A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handhabung Avoid freeze and thaw cycles.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Haltbarkeit 12 months
Allgemeine Veröffentlichungen Stapleton, Carlson, Brokstein et al.: "A Drosophila full-length cDNA resource." in: Genome biology, Vol. 3, Issue 12, pp. RESEARCH0080, 2003 (PubMed).

Ueno, Ohta, Morita et al.: "Trehalose sensitivity in Drosophila correlates with mutations in and expression of the gustatory receptor gene Gr5a." in: Current biology : CB, Vol. 11, Issue 18, pp. 1451-5, 2001 (PubMed).

Ishimoto, Matsumoto, Tanimura: "Molecular identification of a taste receptor gene for trehalose in Drosophila." in: Science (New York, N.Y.), Vol. 289, Issue 5476, pp. 116-9, 2000 (PubMed).

Adams, Celniker, Holt et al.: "The genome sequence of Drosophila melanogaster." in: Science (New York, N.Y.), Vol. 287, Issue 5461, pp. 2185-95, 2000 (PubMed).