GFP Antikörper

Produktnummer ABIN350304

Produktdetails anti-GFP Antikörper

Target: GFP (Green Fluorescent Protein (GFP))

Reaktivität: Alle Spezies

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Specific for EGFP/GFP/Venus

Immunogen: Synthetic peptides (a pentamer) from EGFP/GFP/Venus conjugated to an immunogenic carrier protein was used as the antigen.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: A working concentration of 5-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Abstract: GFP Produkte

Synonyme: green fluorescent protein antikoerper, gfp antikoerper

Substanzklasse: Viral Protein

Hintergrund: GFP is a  naturally fluorescent protein, discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of aequorin’s blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Also known as: (Enhanced) Green Fluorescence Protein.