Cofilin 1 (Non-Muscle) (CFL1) (pY140S156) Antikörper

Antigen: Cofilin 1 (Non-Muscle) (CFL1)

Synonyme: CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, DmelCG4254, CG4254, COFILIN

Epitope: pY140S156

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350237

Produktbeschreibung

Weitere Bezeichnung: Cofilin-1 (Cofilin, non-muscle isoform) (phospho Y140S156)

Swiss-Prot: P23528

Immunogen: A synthetic peptide from the phospho Y140 & S156 of human Cofilin-1 (Cofilin, non-muscle isoform) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and monkey.

Format: Lyophilized

Beschreibung: FUNCTION: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. SUBCELLULAR LOCATION: Nucleus matrix. Cytoplasm, cytoskeleton. Note: Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide. TISSUE SPECIFICITY: Widely distributed in various tissues. Also known as: Cofilin, non-muscle isoform.

Spezifität: Appears to be specific for Cofilin-1.

Synonyme: CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, DmelCG4254, CG4254, COFILIN

Anwendungen

Applikationshinweise: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. This antibody may also recognice the unphosphorylated form. Not tested in other applications.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Ogawa, Tashima, Yumoto et al.: "Coding sequence of human placenta cofilin cDNA." in: Nucleic acids research, Vol. 18, Issue 23, pp. 7169, 1991 (PubMed).

Davidson, Haslam: "Dephosphorylation of cofilin in stimulated platelets: roles for a GTP-binding protein and Ca2+." in: The Biochemical journal, Vol. 301 ( Pt 1), pp. 41-7, 1994 (PubMed).

Nakano, Kanai-Azuma, Kanai et al.: "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family." in: Experimental cell research, Vol. 287, Issue 2, pp. 219-27, 2003 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Rikova, Guo, Zeng et al.: "Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." in: Cell, Vol. 131, Issue 6, pp. 1190-203, 2007 (PubMed).