V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS) Antikörper

Antigen: V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog (FOS)

Synonyme: AP-1, C-FOS, FOS, MGC139881, LOC100222961, cb1065, zgc:77885

Klonalität: Polyklonal

Wirt: Schaf

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN350206

Produktbeschreibung

Weitere Bezeichnung: cFos (c-fos)

Swiss-Prot: P01100

Immunogen: A synthetic peptide from human cFos protein conjugated to an immunogenic carrier protein has been used as the immunogen. The antigen is homologous in many species including rat, mouse, hamster and cat.

Format: Lyophilized

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. SUBUNIT: Heterodimer. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB SUBCELLULAR LOCATION: Nucleus. INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury. SIMILARITY: Belongs to the bZIP family. Fos subfamily SIMILARITY: Contains 1 bZIP domain Also known as: Proto-oncogene protein cFOS.

Spezifität: Appears to be specific for cFos.

Anwendungen

Applikationshinweise: IHC, WB. Use at a concentration of 10-50 µg/ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

Reinheit: IgG

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Roux, Verrier, Klein et al.: "Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells." in: Oncogene, Vol. 6, Issue 11, pp. 2155-60, 1991 (PubMed).

Nakabeppu, Nathans: "The basic region of Fos mediates specific DNA binding." in: The EMBO journal, Vol. 8, Issue 12, pp. 3833-41, 1990 (PubMed).

Hai, Liu, Coukos et al.: "Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers." in: Genes & development, Vol. 3, Issue 12B, pp. 2083-90, 1990 (PubMed).

van Straaten, Mueller, Curran et al.: "Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, Issue 11, pp. 3183-7, 1983 (PubMed).

Glover, Harrison: "Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA." in: Nature, Vol. 373, Issue 6511, pp. 257-61, 1995 (PubMed).

Heilig, Eckenberg, Petit et al.: "The DNA sequence and analysis of human chromosome 14." in: Nature, Vol. 421, Issue 6923, pp. 601-7, 2003 (PubMed).

Vasilescu, Zweitzig, Denis et al.: "The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells." in: Journal of proteome research, Vol. 6, Issue 1, pp. 298-305, 2007 (PubMed).