ASIC1 Antikörper (Extracellular Domain)

Produktnummer ABIN350049

Produktdetails anti-ASIC1 Antikörper

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Bindungsspezifität: Extracellular Domain

Reaktivität: Ratte

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser ASIC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Specific for ASIC1.

Kreuzreaktivität: Human, Maus, Ratte

Kreuzreaktivität (Details): Other species not yet tested.

Aufreinigung: IgG

Immunogen: A synthetic peptide from the extracellular domain of rat ACCN2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and shares 96% identity with human's.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handhabung: Avoid freeze and thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-ASIC1 Antikörper (ABIN350049)

Sudarikova, Bychkov, Kulbatskii, Chubinskiy-Nadezhdin, Shlepova, Shulepko, Koshelev, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Lung Adenocarcinoma Growth and Migration by Selective Interaction With ASIC1/α-ENaC/γ-ENaC Heterotrimer." in: Frontiers in oncology, Vol. 12, pp. 904742, (2022) (PubMed).

Bychkov, Kirichenko, Shulepko, Mikhaylova, Kirpichnikov, Lyukmanova: "Mambalgin-2 Inhibits Growth, Migration, and Invasion of Metastatic Melanoma Cells by Targeting the Channels Containing an ASIC1a Subunit Whose Up-Regulation Correlates with Poor Survival Prognosis." in: Biomedicines, Vol. 9, Issue 10, (2021) (PubMed).

Details zu ASIC1

Target: ASIC1 (ACCN2) (Amiloride-Sensitive Cation Channel 2, Neuronal (ACCN2))

Andere Bezeichnung: ACCN2 (ACCN2 Produkte)

Synonyme: ACCN2 antikoerper, ASIC antikoerper, BNaC2 antikoerper, Accn2 antikoerper, ASIC1 antikoerper, AI843610 antikoerper, ASIC1a antikoerper, B530003N02Rik antikoerper, acid sensing ion channel subunit 1 antikoerper, acid-sensing (proton-gated) ion channel 1 antikoerper, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1 antikoerper, ASIC1 antikoerper, Asic1 antikoerper, asic1 antikoerper, SMARCD1 antikoerper

Hintergrund: This gene encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this gene is expressed in most if not all brain neurons, and it may be an ion channel subunit, however, its function as an ion channel remains unknown. FUNCTION: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). SUBCELLULAR LOCATION: Cell membrane, Multi-pass membrane protein. Note=Localizes in synaptosomes at dendritic synapses of neurons. TISSUE SPECIFICITY: Expressed in dorsal root ganglia (DRG) and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal formation, habenula, basolateral amygdaloid nuclei, and in the Purkinje and granule cells of the cerebellum. Diffusely detected over most other regions of the basal ganglia, including thalamic nuclei, substantia nigra, striatum and globus pallidus, hypothalamus, midbrain, pons, medulla and choroid plexus. Isoform 3 is expressed only in dorsal root ganglion while isoform 1 is expressed in DRG, spinal chord, trigeminal ganglia and the trigeminal mesencephalic nucleus.,ACCN Channels,Acid-sensing ion channel 1, ASIC1, Brain sodium channel 2, BNaC2, ACCN2

UniProt: P55926