GABA(A) Receptor-Associated Protein (GABARAP) Antikörper

Antigen: GABA(A) Receptor-Associated Protein (GABARAP)

Synonyme: MM46, FLJ25768, MGC120154, MGC120155, mg:bb02b03, GABARAP, MGC128374

Klonalität: Polyklonal

Wirt: Schaf

Reaktivität: Human, Ratte (Rattus), Maus

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN349992

Produktbeschreibung

Weitere Bezeichnung: GABA(A) receptor-associated protein (GABARAP, MM46, FLC3B)

Swiss-Prot: O95166

Immunogen: A synthetic peptide as a part of human, rat, mouse GABA(A) receptor-associated protein (GABARAP, MM46, FLC3B) conjugated to immunogenic carrier protein has been used as the immunogen.

Format: Lyophilized

Beschreibung: Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly.autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels ofautophagy have been described in some malignant tumors, and a role forautophagy in controlling the unregulated cell growth linked to cancer has been proposed. GABARAP belongs ot a large family of proteins that mediate intracellular membrane trafficking and/or fusion. GABARAP binds not only to GABA, type A but also to tubulin, gephrin, and ULK1. May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. It interacts with GPHN and NSF; interacts with GABRG2, beta-tubulin and ULK1. SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoplasm, cytoskeleton. Note: Largely associated with intracellular membrane structures including the Golgi apparatus and post-synaptic cisternae. Colocalizes with microtubules. TISSUE SPECIFICITY: Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas. Also known as: GABA(A) receptor-associated protein, MM46, GABARAP, FLC3B, HT004, FLJ25768, MGC120154, MGC120155.

Spezifität: Appears to be specific for GABARAP.

Anwendungen

Applikationshinweise: IHC, WB. Use at a dilution of 1 : 200 to 1 : 5000. The optimal dilution should be determined by the end user.

Reinheit: whole serum

Lagerung: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Forschungsgebiet: Autophagie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Paz, Elazar, Fass: "Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p." in: The Journal of biological chemistry, Vol. 275, Issue 33, pp. 25445-50, 2000 (PubMed).

Kneussel, Haverkamp, Fuhrmann et al.: "The gamma-aminobutyric acid type A receptor (GABAAR)-associated protein GABARAP interacts with gephyrin but is not involved in receptor anchoring at the synapse." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8594-9, 2000 (PubMed).

Tanida, Komatsu, Ueno et al.: "GATE-16 and GABARAP are authentic modifiers mediated by Apg7 and Apg3." in: Biochemical and biophysical research communications, Vol. 300, Issue 3, pp. 637-44, 2002 (PubMed).

Sou, Tanida, Komatsu et al.: "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16." in: The Journal of biological chemistry, Vol. 281, Issue 6, pp. 3017-24, 2006 (PubMed).

Boileau, Pearce, Czajkowski: "Tandem subunits effectively constrain GABAA receptor stoichiometry and recapitulate receptor kinetics but are insensitive to GABAA receptor-associated protein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 49, pp. 11219-30, 2005 (PubMed).

Luo, Kranzler, Zuo et al.: "CHRM2 variation predisposes to personality traits of agreeableness and conscientiousness." in: Human molecular genetics, Vol. 16, Issue 13, pp. 1557-68, 2007 (PubMed).