Aquaporin, Plasma Membrane Intrinistic Protein 2-5 (PIP2,5) Antikörper

Antigen: Aquaporin, Plasma Membrane Intrinistic Protein 2-5 (PIP2,5)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Reis (Oryza sativa)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349691

Produktbeschreibung

Immunogen: KLH-conjugated peptide derived from Oryza sativa PIP2-5 Q8GRI8

Format: Serum

Beschreibung: PIP2,5 protein is an aquaporins which facilitates the transport of water and smallneutral molecules across cell membrane.

Spezifität: Predicted reactivity: monocots including: Sorghum bicolor, Zea mays.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

5ug of crude membrane fraction/lane from Oryza sativa L. cv. Akitakomachi leaf bladeand root were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min.at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milkpowder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T,each time in a fresh polystyrene box and probed with anti-PIP2,5 antibodies (ABIN349691,1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation. Peptide used to elicti this antibody has been added to primary antibody incubation (rightpanel, + +) and therefore specific signal has been depleted in thisneutralization/immunocompetition assay.

Publikationen

Publikationen: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).