Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3) Antikörper

Antigen: Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Reis (Oryza sativa)

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349687

Produktbeschreibung

Immunogen: peptide derived from Oryza sativa PIP1-3 Q9SXF8

Format: Serum

Beschreibung: PIP1,3 is a plasma membrane aquaporine which facilitates trasport of wateracross cell membrane. Alternative names: aquaporin PIP 1-3, water channelprotein RWC3, RWC-3

Spezifität: Predicted reactivity: monocots including: Hordeum vulgare, Stipa sp. Triticum aestivum.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1.5ug of crude membrane fraction/lane from Oryza sativa L.Akitakomachi were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer).Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05%Triton X.100). Membranes were washed 5 times with TBS-T, each timein a fresh polystyrene box and probed with anti-PIPs1,3 antibodies(ABIN349687, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). Allsteps were performed in RT with agitation. Below: Immunospecificity of anti.PIP1,3 antibodies to recombinant PIPproteins expressed in yeast cells. 1.5ug protein/lane. Most aquaporins show the dimerc forms in addition to monomer evenin the presence of SDS.

anti-Aquaporin, Plasma Membrane Intrinistic Protein 1-3 (PIP1,3) antibody (Image 2)

Publikationen

Publikationen: Sakurai, Ahamed, Murai et al.: "Tissue and cell-specific localization of rice aquaporins and their water transport activities." in: Plant & cell physiology, Vol. 49, Issue 1, pp. 30-9, 2008 (PubMed).