ATPase, H+ Transporting, Lysosomal V1 Subunit A (ATP6V1A) Antikörper

Antigen: ATPase, H+ Transporting, Lysosomal V1 Subunit A (ATP6V1A)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Acetabularia, Barley (Hordeum vulgare), Reis (Oryza sativa), Pinus sylvestris, Pyrus, Ricinus communis

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349685

Produktbeschreibung

Weitere Bezeichnung: Vacuolar H+-ATPase subunit A (V-ATPase, A)

Immunogen: purified native V-ATPase subunit A from Ricinus communis

Format: Serum

Beschreibung: V-ATPase subunit A is a catalytic subunit of V1 complex of vacuolar ATPase.This enzyme (EC=3.6.3.14) is involved in acidification process of variouscompartements of eucaryotic cell. This protein is coded by VHA-A gene.Alternative names: Vacuolar proton pump subunit alpha, vacuolar H(+)-ATPasesubunit A, V-ATPase 69 kDa subunit

Spezifität: Predicted reactivity: dicots including: Arabidopsis thaliana, Gossypium mexicanum, Vitis vinifera,monocots including: Hordeum vulgare, trees: Populus trichocarpa.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

65ug/lane of purified vacuolar membranes from Vigna radiata L. (1,3) and purifiedV-ATPase, 7.4ug/lane (2,4) were separated on 12 % SDS-PAGE and blotted 1h toPVDF membrane (40 min. at 10 V using BioRad semidry transfer). Filters wereblocked 1h with 5 % low-fat milk powder in TBS-T (0.05% Triton X.100). Membraneswere washed 5 times with TBS-T, each time in a fresh polystyrene box and probedwith anti-V-ATPase subunit A antibodies (ABIN349685, 1:2000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. CBB - staining with Coomasie blue - left panel. Immunoblot - western blot detectoin -right panel.

Publikationen

Publikationen: Smart, Vojdani, Maeshima et al.: "Genes involved in osmoregulation during turgor-driven cell expansion of developing cotton fibers are differentially regulated." in: Plant physiology, Vol. 116, Issue 4, pp. 1539-49, 1998 (PubMed).

Kawamura, Arakawa, Maeshima et al.: "Tissue specificity of E subunit isoforms of plant vacuolar H(+)-ATPase and existence of isotype enzymes." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6515-22, 2000 (PubMed).

Matsuura-Endo, Maeshima, Yoshida: "Mechanism of the Decline in Vacuolar H -ATPase Activity in Mung Bean Hypocotyls during Chilling." in: Plant physiology, Vol. 100, Issue 2, pp. 718-722, 2006 (PubMed).