Probable Aquaporin SIP2-1 Antikörper

Antigen: Probable Aquaporin SIP2-1

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349680

Produktbeschreibung

Weitere Bezeichnung: Probable aquaporin SIP2-1 (AtSIP2,1)

Immunogen: KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana SIP2,1Q9M1K3

Format: Serum

Beschreibung: SIP protein functions as a water channel to facilitate the transport of water acrosscell membrane. It is mainly localized to ER.Alternative name: small basic intrinisticprotein 2-1

Spezifität: Predicted reactivity: not reactive in.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

crude membrane fraction/lane from Arabidopsis thaliana stems (1,3) and crudemembrane fraction from yeast cells expressing SIP2,1 (2,4) were separated on 12 %SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRadsemidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in afresh polystyrene box and probed with anti-AtSIP2,1 antibodies (ABIN349680, 1:1000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation. Peptide used to elicit anti-SIP2,1 antibodies have been incubated with antibodies -right panel.

anti-Probable Aquaporin SIP2-1 antibody (Image 2)

Publikationen

Publikationen: Ishikawa, Suga, Uemura et al.: "Novel type aquaporin SIPs are mainly localized to the ER membrane and show cell-specific expression in Arabidopsis thaliana." in: FEBS letters, Vol. 579, Issue 25, pp. 5814-20, 2005 (PubMed).