Tonoplast Intrinistic Protein 1-1, 1-2 (Gamma) (TIP1,1, TIP1,2) Antikörper

Antigen: Tonoplast Intrinistic Protein 1-1, 1-2 (Gamma) (TIP1,1, TIP1,2)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349677

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1,1 andTIP1,2 (protein accesion number available in Suga et al. 2001). Peptide is alsoconserved in Arabidopsis thaliana TIP1-1 P25818, TIP1-2 Q41963

Format: Serum

Beschreibung: TIPs belong to MIP/aquaporin protein family.Alternative names: AquaporinTIP1-1,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP,gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsicprotein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, saltstress-induced tonoplast intrinsic protein

Spezifität: Predicted reactivity: dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis,monocots including: Hordeum vulgare, Oryza sativa, trees: Populus trichocarpa.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-TIP1s antibodies (ABIN349659, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation. TIP proteins usually show a faint band of dimeric form at 42 kDa in addition to the majormonomer band of 23 kDa even in the presence of SDS.

Publikationen

Publikationen: Suga, Imagawa, Maeshima: "Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs." in: Planta, Vol. 212, Issue 2, pp. 294-304, 2001 (PubMed).