Plasma Membrane Intrinistic Protein 2-1,2-2, 2-3 Antikörper

Antigen: Plasma Membrane Intrinistic Protein 2-1,2-2, 2-3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349675

Produktbeschreibung

Weitere Bezeichnung: Plasma membrane intrinistic protein 2-1,2-2, 2-3 (PIP2,1,PIP2,2,PIP2,3)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana PIP2proteins: AtPIP2-1, AtPIP2-2 P43287, AtPIP2-3 P30302

Format: Serum

Beschreibung: PIP2,2 is a plasma membrane aquaporin. Alternative names of isoforms:aquaporin PIP2-1, plasma membrane intrinsic protein 2a, PIP2a, aquaporinPIP2-2, plasma membrane intrinsic protein 2b, PIP2b, TMP2b, Aquaporin PIP2-3,plasma membrane intrinsic protein 2c, PIP2c, TMP2C, RD28-PIP, waterstress-induced tonoplast intrinsic protein, (WSII-TIP)

Spezifität: Predicted reactivity: dicots including: Brassica napus, Gossypium hirsutum, Ricinus communis,monocots including: Hordeum vulgare Oryza sativa, Zea mays, trees: Populustremula x Populus tremloides, moss: Physcomitrella patens.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2s antibodies (ABIN349675, 1:1000, 1h) and secondaryanti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.