Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3) Antikörper

Antigen: Aquaporins (PIP1,1, PIP1,2, PIP1,3) (PIP1,1/PIP1,2/PIP1,3)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349673

Produktbeschreibung

Weitere Bezeichnung: Aquaporins (PIP1,1, PIP1,2, PIP1,3)

Immunogen: KLH-conjugated synthetic peptide derived from N terminus of Raphanussativus PAQ1O80368. Chosen peptide is conserved in PIP1,1, PIP1,2, PIP1,3N-terminus.

Format: Serum

Beschreibung: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane.

Spezifität: Predicted reactivity: dicots including: Lycopersicum esculentum, Ricinus communis, monocotsincluding: Hordeum vulgare, Oryza sativa, Zea mays, trees: Populus trichocarpa.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 Vusing BioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powderin TBS-T (0.05% Triton X.100). Membranes were washed 5 times with TBS-T, eachtime in a fresh polystyrene box and probed with anti-PIP1s ntibodies (diluted in 1: 500(left panel) and 1: 1000 (right panel), ABIN349673, and secondary anti-rabbit (1:2000, 1h). All steps were performed in RT with agitation.