Aquaporin PIP2,1 (PIP2,1) Antikörper

Antigen: Aquaporin PIP2,1 (PIP2,1)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349672

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Raphanus sativus PAQ2 O80369

Format: Serum

Beschreibung: PIPs proteins are aquaporins which facilitate the transport of water and smallneutral molecules across cell membrane.

Spezifität: Predicted reactivity: dicots including: Brassica napus, Gossypium mexicanum.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2,1 antibodies (ABIN349672, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publikationen

Publikationen: Geisler, Girin, Brandt et al.: "Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters." in: Molecular biology of the cell, Vol. 15, Issue 7, pp. 3393-405, 2004 (PubMed).