Calmodulin-Stimulated Calcium-ATPase (Ca2+ ATPase) Antikörper

Antigen: Calmodulin-Stimulated Calcium-ATPase (Ca2+ ATPase)

Synonyme: Ca-ATPase, Ca-p, Ca-P60, CA-P60A, CaP60A, Ca[2+]ATPase, CG3725, DmelCG3725, dSERCA, Kum, l(2)k08308ab, l(2)k09025, SERCA, SERCA2, ATP2A2, SERCA2A

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Blumenkohl (Brassica oleracea), Raphanus sativus

Applikation: Western Blot (WB), ELISA

Produktnummer: ABIN349670

Produktbeschreibung

Weitere Bezeichnung: Calmodulin-stimulated calcium-ATPase (Ca2+-ATPase)

Immunogen: KLH-conjugated synthetic peptide derived from Brassica oleraceacalmodulin-stimulated calcium-ATPase P93067

Format: Serum

Beschreibung: Ca2+-ATPase | calmodulin-stimulated calcium-ATPase belongs to family ofcatoin transport ATPase (P-type).

Spezifität: Predicted reactivity: dicots including: Vitis vinifera, monocots including: Oryza sativa, Triticumaestivum, trees: Populus balsamifera, moss: Physcomitrella patens.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. This protein is of low abundance in plant tissues. Protocol for tonoplast membrane isolation can be found here.

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Raphabus sativa L.Tokinashi-daikon were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane(40 min. at 10 V using BioRad semidry transfer). Filters were blocked 1h with 5 % low-fatmilk powder in TBS-T (0.05% Triton X.100). Membranes were washed 5 times withTBS-T, each time in a fresh polystyrene box and probed with anti-Ca2+ ATPase antibodies (ABIN349670, 1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps wereperformed in RT with agitation.