Vacuolar Zn2+/H+ Antiporter (AtMTP1) Antikörper

Antigen: Vacuolar Zn2+/H+ Antiporter (AtMTP1)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349669

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana MTP1Q9ZT63

Format: Serum

Beschreibung: MTP1 is mediating zinc accumulation in roots and resistance to zinc. Is involved insequestering of zinc from cytoplasm into vacuole in conditions of its excess.Alternative names: Zinc transporter ZAT-1, ZAT1p, 43.8 | metal tolerance protein1

Spezifität: Predicted reactivity: dicots including: Brassica sp. , Noccaea fendleri, Thlaspi caerulescens.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. MTP1 protein is of low abundance in plant tissues. Protocol for isolation of tonoplast proteins can be found here.

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

sample of a vector (1) and vacuolar membrane fraction of yeast cells expressing AtMTP1 (2)separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-AtMTP1 antibodies (ABIN349669, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publikationen

Publikationen: Kobae, Uemura, Sato et al.: "Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis." in: Plant & cell physiology, Vol. 45, Issue 12, pp. 1749-58, 2005 (PubMed).

Kawachi, Kobae, Mimura et al.: "Deletion of a histidine-rich loop of AtMTP1, a vacuolar Zn(2+)/H(+) antiporter of Arabidopsis thaliana, stimulates the transport activity." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8374-83, 2008 (PubMed).