Metal Tolerance Protein B (AtMTP4) Antikörper

Antigen: Metal Tolerance Protein B (AtMTP4)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349660

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana Q6DBM8

Format: Serum

Beschreibung: MTP4 protein is involved in maintaining zinc homeostasis by sequestration ofexcess zinc in the cytoplasm into vacuole. Alternative name: AtMTPb

Spezifität: Predicted reactivity: dicots including: Capsella rubella.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

5ug of crude membrane fraction from Arabidopsis thaliana incubated with peptideused to elicit anti-AtMTP4 antibodies (1), 5ug of crude membrane fraction fromArabidopsis thaliana (2), His tag recombinant MTP4 protein (3), were separated on 12 %SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V using BioRad semidrytransfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T (0.05% TritonX.100). Membranes were washed 5 times with TBS-T, each time in a fresh polystyrenebox and probed with anti-AJ011604 | nitrate transporter antibodies (ABIN349660 1:1000, 1h)and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.