Nitrate Transporter (AJ011604) Antikörper

Antigen: Nitrate Transporter (AJ011604)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349659

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana nitratetransporter Q9SZY4

Format: Serum

Beschreibung: Nitrate transporter functions in transporter activity in response to jasmonic acidstimulus and response to wounding. This protein is coded by ntp2 gene.

Spezifität: Predicted reactivity: Arabidopsis thaliana, Raphanus sativus.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 1000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. This protein is present in plant tissues in very low levels. Protocol for isolation of plant vacuolar membranes can be found here.

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

5ug and 10ug of vacuolar membrane fraction/lane from Raphanus sativus wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-AJ011604 | nitrate transporter antibodies (ABIN349659,1:1000, 1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT withagitation.