Plasma Membrane Aquaporin (PIP2-7) Antikörper

Antigen: Plasma Membrane Aquaporin (PIP2-7)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349656

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thalianaPIP2,7,P93004

Format: Serum

Beschreibung: Plasma membrane aquaporin, PIP2,7 is water channel protein required forwater transport across cell membrane. Alternative names: plasma membraneintrinsic protein 2-7, AtPIP2,7, plasma membrane intrinsic protein 3, saltstress-induced major intrinsic protein, PIP3a

Spezifität: Predicted reactivity: dictos including: Brassica oleracea, Spinacia oleracea, Vitis vinifera,trees: Juglansregia, Populus tremulax Populus tremuloides.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of plasma membranes are used, one µg

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-PIP2,7 antibodies (ABIN349656, 1:1000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.