ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C) Antikörper

Antigen: ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana, Raphanus sativus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN349655

Produktbeschreibung

Weitere Bezeichnung: vacuolar H+-ATPase, subunit c (16 kDa) (V-ATPase, c)

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit c,Q6IDA4

Format: Liquid

Beschreibung: V-ATPase c subunit is located in vacuole and is involved in ATP synthesis cuopled proton transport. This protein is coded by ATVHA-C3 gene. Alternative names: AT4g34720/T4L20_300

Spezifität: Predicted reactivity: dictos including: Gossypium mexicanum, Phaseolus aureus, Rcicinus communis, monocots including: Oryza sativa, Triticum aestivum, Zea mays, trees: Picea sitchensis, Populus trichocarpa. Not reactive in: no confirmed exceptions from predicted reactivity known in the moment.

Anwendungen

Applikationshinweise: Recommended dilution: 1: 8000 (ELISA), 1: 2000 with standard ECL (WB). Molecular Weight: 16 | 16 kDa (Arabidopsis thaliana) (expected, apparent). Additional information: Protein or membrane sample should be treated at 70oC for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20oC to -80oC. Manufactured by Operon Biotechnologies.

Buffer: serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug, 5 and 10ug of vacuolar membrane fraction/lane from Raphanus sativus were separated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-V-ATPase c subunit antibodies (ABIN349655, 1:1000,1h) and secondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

anti-ATPase, H+ Transporting, Lysosomal V1 Subunit C (ATP6V1C) antibody (Image 2)

Publikationen

Publikationen: Kobae, Mizutani, Segami et al.: "Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells." in: Bioscience, biotechnology, and biochemistry, Vol. 70, Issue 4, pp. 980-7, 2006 (PubMed).