Vacuolar H+-Pyrophosphatase (V-PPase) Antikörper

Antigen: Vacuolar H+-Pyrophosphatase (V-PPase)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Arabidopsis thaliana

Applikation: ELISA, Western Blot (WB), Immunolokalisation (IL)

Produktnummer: ABIN349652

Produktbeschreibung

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,P31414

Format: Serum

Beschreibung: V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1),EC=3.6.1.1, acts to establish proton gradient of often higher magnitude thanH+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1,H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar protonpyrophosphatase 3

Spezifität: Predicted reactivity: dicots including: Nicotiana tabacum, Ricinus communis, Vitis vinifera, Saccharumofficinarum, monocots including: Horderum vulgare, Oryza sativa, Zea mays,trees: Picea sitchensis, Populus trichocarpa.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 8000 (ELISA), 1: 2000 with standard ECL (WB), 1: 100 (IL).

Buffer: 0.1 % sodium azide is added as preservative. For antibody re-suspendinginformation check the tube lable. Antibodies will detect target protein in a few µg of a crude preparation loaded perwell. If purified preparations of vacuolar and plasma membranes are

Lagerung: store at -20°C, make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin tubes briefly prior to opening them to avoid any losses thatmight occur from material adhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

1ug and 10ug of crude membrane fraction/lane from Arabidopsis thaliana wereseparated on 12 % SDS-PAGE and blotted 1h to PVDF membrane (40 min. at 10 V usingBioRad semidry transfer). Filters were blocked 1h with 5 % low-fat milk powder in TBS-T(0.05% Triton X.100). Membranes were washed 5 times with TBS-T, each time in a freshpolystyrene box and probed with anti-V-PPase antibodies (ABIN349652, 1:2000, 1h) andsecondary anti-rabbit (1:2000, 1 h). All steps were performed in RT with agitation.

Publikationen

Publikationen: Kobae, Mizutani, Segami et al.: "Immunochemical analysis of aquaporin isoforms in Arabidopsis suspension-cultured cells." in: Bioscience, biotechnology, and biochemistry, Vol. 70, Issue 4, pp. 980-7, 2006 (PubMed).

Kabaa, Janicka-Russak: "Differential regulation of vacuolar H+-ATPase and H+-PPase in Cucumis sativus roots by zinc and nickel." in: Plant science : an international journal of experimental plant biology, Vol. 180, Issue 3, pp. 531-9, 2011 (PubMed).