Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5) Antikörper

Antigen: Solute Carrier Family 7 (Cationic Amino Acid Transporter, Y+ System), Member 5 (SLC7A5)

Synonyme: E16, TA1, SLC7A5, LAT-1, slc7a5a, 4F2LC, D0H16S474E, JHI-21, LAT1

Klonalität: Polyklonal

Wirt: Bitte anfragen

Applikation: Western Blot (WB), Immunhistochemie (IHC)

Produktnummer: ABIN348944

Produktbeschreibung

Weitere Bezeichnung: SLC7A5/LAT1 (poly)

Immunogen: This antibody was affinity purified from rabbit serum. The rabbit was immunized with a synthetic peptide derived from human SLC7A5.

Beschreibung: SLC7A5, L-type amino acid transporter 1 (LAT1), forms as a heterodimeric complex with the heavy chain of the cell surface antigen 4F2 (4F2hc/CD98). It transports neutral amino acids, most of which are essential amino acids across blood brain barrier. L-dopa, which is commonly used to treat Parkinson's disease, also enters the brain the LAT1/CD98 complex. In addition, tumors with increased expression of SLC7A5 have been reported. Increased uptake of amino acids through SLC7A5 may facilitate tumor cell growth and survival.

Spezifität: This antibody can be used to stain endogenous antigen in paraffin embedded human tissues including brain by immunohistochemistry. The reactivity of this antibody has been confirmed by Western blotting to detect the full-length of human SLC7A5 transiently expressed in HEK 293T cells.

Anwendungen

Applikationshinweise: Western blotting, 1:1,000 for chemiluminescence detection system Immunoprecipitation, Not tested Immunohistochemistry, 1:1,000 Heat treatment is necessary for staining paraffin embedded sections. Autoclave, 125 o C for 5 minutes in 10 mM citrate buffer containing 0.05% Tween-20 (pH 6.0). Immunocytochemistry, Not tested Flow cytometry, Not tested . Protocol: Immunohistochemical staining for paraffin-embedded sections 1) Deparaffinize the sections with Xylene 3 times for 3-5 minutes each. 2) Wash the slides with Ethanol 3 times for 3-5 minutes each. 3) Wash the slides with PBS 3 times for 3-5 minutes each. 4) Heat treatment by Autoclave: Heat the slides immersed in retrieval solution [10 mM citrate buffer containing 0.05% Tween-20 (pH 6.0)] at 125oC for 5 minutes in pressure boiler. After boiling, the slides should remain in the pressure boiler until the brain Immunohistochemical detection of SLC7A5 on paraffin embedded section of human brain with BMP011. Tissue array (MBL, code no. COM01003) was used for this application.

Buffer: 100 uL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained.

Beschränkungen: Nur für Forschungszwecke einsetzbar