C-Abl (pTyr245) Antikörper

Antigen: C-Abl

Epitope: pTyr245

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN348661

Produktbeschreibung

Weitere Bezeichnung: Phospho-c-Abl [Tyr245]

Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl that contains tyrosine 245. Note: there are two widely expressed forms of c-Abl produced by alternative splicing, known as 1a and 1b (the more commonly used form). The corresponding phosphorylation site from 1a is tyrosine 226.

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: C -Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein BCR/ABL. Tyrosine 245 is involved in the activation of c-Abl kinase activity, and phosphorylated by Src after PDGF stimulation.

Spezifität: Human c-Abl. Mouse (92% homologous) c-Abl has not been tested, but is expected to react. APPLICATIONS : The antibody has been used for Western blotting applications. For Western blotting, we recommend using the antibody at 0.1-1.0 ug/mL. At 0.50 ug/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. The optimal antibody concentration should be determined for each specific application.

Anwendungen

Reinigung: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Abl. The final product is generated by affinity chromatography using a c-Abl -derived peptide that is phosphorylated at tyrosine 245.

Buffer: Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg 2+ and Ca 2+ ), pH 7.3 (+/- 0.1), with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.

Beschränkungen: Nur für Forschungszwecke einsetzbar