ERK1/2 MAP Kinase Antikörper (AP)

Antigen: ERK1/2 MAP Kinase

Klonalität: Monoklonal (ERK7D8)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Konjugat: Alkaline Phosphatase (AP)

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (IHC), Elektromobilitäts-Experimente (EMSA)

Produktnummer: ABIN348097

Produktbeschreibung

Weitere Bezeichnung: ERK1+2 (MAP Kinase)

Immunogen: Synthetic Peptide (a.a. 324-345) corresponding to a segment near the C-terminus of rat ERK1 (1)

Isotyp: IgG1 kappa

Klon: ERK7D8

Beschreibung: Mitogen Activated Protein Kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus. These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways. In mammalian cells, the best characterized sub-group of the MAP Kinase family are the Extracellular Signal Regulated Kinases (ERKs). To date, at least 4 distinct ERKs have been identified including: ERK1 (p44/p43), ERK2 (p42/p43), ERK3 (p62), and ERK4 (p45). Analysis of cDNAs encoding MAP Kinase suggests that numerous other ERKs may exist. MAP Kinase has been shown to phosphorylate numerous proteins including: RSK (2) , c-Fos, c-Jun, c-Myc, c-raf, MAP2, and MEK (3) . MAP Kinase is directly activated when phosphorylated by MEK and indirectly stimulated by many factors (4,5,6) .

Spezifität: This antibody reacts strongly with both ERK1 and ERK2. Reactivity has been confirmed by western blot analysis of extracts derived from human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain, and recombinant ERK2.

Anwendungen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. Optimal dilutions will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations. We recommend the following ranges as starting points. ELISA: 0.1-1 ug/ml Immunoprecipitation (7-9, 13, 14) : 2-5 ug/reaction Western Blotting (1-8, 10-19) : 1 ug/ml EMSA: inquire

Buffer: This monoclonal antibody is supplied as a 400 ul aliquot at 0.5 mg/ml in phosphate buffered saline, pH 7.4, containing 0.05% sodium azide. The antibody is highly purified from mouse ascites by peptide-specific affinity chromatography.

Beschränkungen: Nur für Forschungszwecke einsetzbar