E. coli LexA Antikörper

Antigen: E. coli LexA

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Escherichia Coli (E. Coli)

Applikation: Western Blot (WB)

Produktnummer: ABIN348075

Produktbeschreibung

Beschreibung: E. coli LexA protein binds specifically to the SOS-box sequenceand represses the genes belonging to the SOS regulon. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As the results, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced. 1 . The lexA gene is used for yeast two-hybrid experiments as a bate to identify the protein-protein interaction in vivo. 2 .

Anwendungen

Buffer: This product was prepared by immunizing rabbit with full-size highly-purified recombinant LexA protein. Using this antibody, 23 kD LexA protein was identified in the E.coli whole-cell lysate (Fig 1) and the expression of bait constructs was identified in

Beschränkungen: Nur für Forschungszwecke einsetzbar