acetylated-p53 (lys382) Antikörper

Antigen: acetylated-p53 (lys382)

Klonalität: Monoklonal (2B7E4)

Wirt: Bitte anfragen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN347851

Produktbeschreibung

Immunogen: This product is the purified IgG fraction obtained from culture supernatant of mouse hybridoma (clone 2B7E4) which produces monoclonal antibody that specifically recognizes p53 with acetylated at Lys382.

Isotyp: IgG1 kappa

Klon: 2B7E4

Beschreibung: P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53 kDa position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions and stability of p53 are regulated by the phosphorylation of serine and threonine, and the acetylation of lysine at various sites in the molecule. Acetylation of lysine 382 (acetyl-K382) of p53 occurs after DNA damage and is catalyzed by the p300/CBP acetyltransferase, which stabilize p53 protein (ref 1) .

Spezifität: This antibody reacts with human acetylated p53 (Lys382) on Western blotting.

Anwendungen

Applikationshinweise: Western blotting, 0.5-1 ug/mL for chemiluminescence detection system Immunoprecipitation, Not tested Immunocytochemistry, Not tested Immunohistochemistry, Not tested Flow cytometry, Not tested

Konzentration: 1 mg/mL

Buffer: 50 ug IgG in 50 uL volume of PBS containing 50% glycerol, pH 7.2. No preservative is contained. Sterilized by filtration.

Beschränkungen: Nur für Forschungszwecke einsetzbar