Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) Antikörper

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyme: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA

Klonalität: Monoklonal (EM-05)

Wirt: Ratte

Reaktivität: Maus

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB), Immunzytochemie (ICC)

Produktnummer: ABIN343710

Produktbeschreibung

Weitere Bezeichnung: CD45

Immunogen: Murine peripheral blood leukocytes

Format: Purified

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EM-05

Beschreibung: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphataseubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10%of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocytedevelopment and antigen signaling, serving as an important regulator of Src-family kinases.CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoformsdiffer in their extracellular domains, whereas they share identical transmembrane andcytoplasmic domains. These isoforms differ in their ability to translocate into theglycosphingolipid-enriched membrane domains and their expression depends on cell typeand physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 isimportant in promoting cell survival by modulating integrin-mediated signal transductionpathway and is also involved in DNA fragmentation during apoptosis.

Spezifität: The antibody EM-05 reacts with mouse CD45 antigen (Leukocyte Common Antigen), asingle chain type I transmembrane protein expressed at high level on cells of hematopoieticorigin, except erythrocytes and platelets.

Anwendungen

Applikationshinweise: Flow Cytometry: Recommended dilution: 2 µg/ml. Positive control: murine peripheral blood leukocytes

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by hydrophobic charge inductionchromatography followed by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Immunologie, Adaptive Immunität, Hämopoetische Vorläufer, Glia Marker, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Surface staining of C57BL/6 cells and CD45 knock-out cells with anti-CD45 antibodies EM-05 and ALI-4A2.