V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antikörper

Antigen: V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyme: MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746

Klonalität: Monoklonal (IMD-3)

Wirt: Bitte anfragen

Reaktivität: Human

Applikation: Western Blot (WB), Immunzytochemie (ICC), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343589

Produktbeschreibung

Weitere Bezeichnung: c-Myc

Immunogen: Synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG2a

Klon: IMD-3

Beschreibung: C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells, in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol (2ME2)-induced oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in vivo.

Spezifität: Human. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 4 µg/ml with the appropriate system to detect c-Myc in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 8 µg/ml to detect c-Myc in formalin fixed and paraffin embedded tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Forschungsgebiet: Transkriptionsfaktoren, Krebs, Zellzyklus

Beschränkungen: Nur für Forschungszwecke einsetzbar