Heat Shock Protein 70 (HSP70) (HSPA4) Antikörper

Antigen: Heat Shock Protein 70 (HSP70) (HSPA4)

Klonalität: Monoklonal (SJ-70)

Wirt: Bitte anfragen

Reaktivität: Human

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343567

Produktbeschreibung

Weitere Bezeichnung: Heat Shock Protein 70, HSP70

Immunogen: HSP70 isolated from bovine brain. Purification Purified by the goat anti-mouse IgG affinity chromatography. Application

Format: Affinity-purified

Isotyp: IgG1

Klon: SJ-70

Beschreibung: Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. (1989) identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.

Spezifität: Human. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5 µg/ml with the appropriate system to detect HSP70 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1 µg/ml to detect HSP70 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1 µg/ml to detect HSP70 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar