Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2) Antikörper

Antigen: Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2)

Synonyme: HDMX, hdm2, MGC5370, MGC71221, Mdm-2, AA415488, 1700007J15Rik

Klonalität: Monoklonal (Jan 02)

Wirt: Bitte anfragen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunzytochemie (ICC)

Produktnummer: ABIN343559

Produktbeschreibung

Weitere Bezeichnung: MDM2

Immunogen: Synthetic peptide corresponding to amino acids 154-167 of human MDM2 conjugated to KLH. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1

Klon: Jan 02

Beschreibung: The MDM2 gene was originally identified by virtue of its amplification in a spontaneously transformed derivative of mouse BALB/c cells, and the MDM2 protein was subsequently shown to bind to p53 in rat cells transfected with p53 genes . Mdm2 acts as a major regulator of the tumor suppressor p53 by targeting its destruction. MDM2 gene is mapped to 12q14.3-q15. The mdm2 gene encodes a zinc finger protein that negatively regulates p53 function by binding and masking the p53 transcriptional activation domain. Overexpression of the MDM-2 gene in cells increases their tumorigenic potential and overcomes the growth-suppressive activity of p53.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5-1 µg/ml with the appropriate system to detect MDM2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect MDM2 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect MDM2 in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Forschungsgebiet: Krebs, Zellzyklus, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar