N-Cadherin (CD325) Antikörper

Antigen: N-Cadherin (CD325)

Klonalität: Monoklonal (NC-17)

Wirt: Bitte anfragen

Reaktivität: Human, Maus, Ratte (Rattus), Kaninchen, Huhn

Applikation: Western Blot (WB), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343552

Produktbeschreibung

Weitere Bezeichnung: N-Cadherin

Format: Affinity-purified

Isotyp: IgG1

Klon: NC-17

Beschreibung: N-cadherin (NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule (L-CAM). N-Cadherin, also knows as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene (including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Spezifität: Human, mouse, rat, rabbit, chicken. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 2 µg/ml with the appropriate system to detect N-cadherin in cells and tissues. Immunohistochemistry(F) At 4 µg/ml to detect N-cadherin in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar