Nebulin (NEB) Antikörper

Antigen: Nebulin (NEB)

Synonyme: NEM2, NEB177D, FLJ11505, FLJ36536, FLJ39568, FLJ39584, DKFZp686C1456, AI595938, NEB, im:6904860, im:7001777, im:7144538, im:7150492, wu:fc33b04, si:ch211-168m7.5

Klonalität: Monoklonal (Neb-20)

Wirt: Bitte anfragen

Reaktivität: Human, Maus, Ratte (Rattus), Huhn, Frosch

Applikation: Western Blot (WB)

Produktnummer: ABIN343550

Produktbeschreibung

Weitere Bezeichnung: Nebulin

Immunogen: Chicken breast nebulin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1

Klon: Neb-20

Beschreibung: Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Spezifität: Human, mouse, rat, chicken, frog. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2ml with the appropriate system to detect nebulin in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar