Neurofilament 68 NF68 Antikörper

Antigen: Neurofilament 68 NF68

Klonalität: Monoklonal (NF-68)

Wirt: Bitte anfragen

Reaktivität: Human, Schwein

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343549

Produktbeschreibung

Immunogen: Pig spinal cord. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1

Klon: NF-68

Beschreibung: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM) and 200 kD (NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68 (NF68), also called Neurofilament Protein, Light Chain (NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E (CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.

Spezifität: Human, pig. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2 µg/ml with the appropriate system to detect NF68 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 2-4 µg/ml to detect NF68 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 2-4 µg/ml to detect NF68 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar