Neurofilament 200 (Phos. and Nonphos.) NF200 Antikörper

Antigen: Neurofilament 200 (Phos. and Nonphos.) NF200

Klonalität: Monoklonal (NF-200)

Wirt: Bitte anfragen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343548

Produktbeschreibung

Immunogen: C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1

Klon: NF-200

Beschreibung: Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD (NFL), 125 kD (NFM), and 200 kD (NFH) on SDS-gel electrophoresis. Genomic clones for the largest human neurofilament protein (NF-H) were isolated, the intron/exon boundaries mapped and the entire protein-coding regions (exons) sequenced. mutations in neurofilaments have been linked to some forms of Charcot-Marie-Tooth disease (CMT).

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5ml with the appropriate system to detect NF200 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2 µg/ml to detect NF200 in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 1-2 µg/ml to detect NF200 in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar