Protein Kinase Ba Antikörper

Antigen: Protein Kinase Ba

Klonalität: Monoklonal (IML-26)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Huhn

Applikation: Western Blot (WB)

Produktnummer: ABIN343534

Produktbeschreibung

Immunogen: Synthetic peptide corresponding to amino acids 461-477 of human PKBa/Akt1. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: IML-26

Beschreibung: PKBaalso knows as V-AKT murine thymoma vial oncogene homolog 1(ATK1). AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and is abrogated by mutations in the pleckstrin homology domain of AKT1. AKT1 gene is mapped to chromosome 14q32.3. Akt1/protein kinase B-alpha is critical for ischemic and VEGF-mediated angiogenesis. Akt1 regulates pathological angiogenesis, vascular maturation and permeability in vivo.

Spezifität: Human, mouse, rat, chicken. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.25-0.5 µg/ml with the appropriate system to detect PKBa in cells and tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar