Tropomyosin(36kD) Antikörper

Antigen: Tropomyosin(36kD)

Klonalität: Monoklonal (TM-36)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Huhn

Applikation: Western Blot (WB)

Produktnummer: ABIN343523

Produktbeschreibung

Immunogen: Chicken gizzard tropomyosin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: TM-36

Beschreibung: The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes, in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.

Spezifität: Human, mouse, rat, chicken. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5-1µg/ml with the appropriate system to detect tropomyosin in cells and tissues. Immunohistochemistry(F) At 1-2µg/ml to detect tropomyosin in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar