Troponin T Antikörper

Antigen: Troponin T

Synonyme: BcDNA:LD08591, DmTpnT, FBgn0004169, TnT, TpnT, dro TnT, int, upheld2, wupB, DmelCG7107, CG7107

Klonalität: Monoklonal (TT-98)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Kaninchen, Huhn

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343521

Produktbeschreibung

Immunogen: Troponin T from rabbit skeletal muscle. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: TT-98

Beschreibung: The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle (TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle (TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.

Spezifität: Human, mouse, rat, rabbit, chicken. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 2-4µg/ml with the appropriate system to detect Troponin T in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 4-8µg/ml to detect Troponin T in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 4-8µg/ml to detect Troponin T in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Forschungsgebiet: Serum/Plasma Proteine

Beschränkungen: Nur für Forschungszwecke einsetzbar