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Vimentin (VIM) Antikörper

Antigen

Vimentin (VIM)

Synonyme FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime
Klonalität Monoklonal (VMT-24)
Wirt
Reaktivität
Alternativen

Human, Maus, Ratte (Rattus)

Applikation
Alternativen Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))
Produktnummer ABIN343517
Menge 100ug
Preis 217,95 €   Zzgl. Versandkosten €20,00 und MWSt
Lieferung nach
Verfügbarkeit Lieferung in 7 bis 10 Werktagen

Produktbeschreibung

Weitere Bezeichnung Vimentin
Immunogen Pig eye lens vimentin. Purification Purified by the goat anti-mouse IgG affinity chromatography.
Format Affinity-purified
Isotyp IgG1  (Passende Sekundärantikörper)
Klon VMT-24
Beschreibung The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. Vimentin gene express in human lymphocytes and in Burkitt's lymphoma cells. Vimentin is secreted by activated macrophages. The gene encoding human vimentin is located on the short arm of chromosome 10.
Spezifität Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise Western blotting: At 0.5-1µg/ml with the appropriate system to detect vimentin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1.5-2.5µg/ml to detect vimentin in formalin fixed and paraffin embedded tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.
Buffer Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.
Lagerung At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.
Forschungsgebiet Abstammungsmarker, Zytoskelett, Neuronale Stammzellmarker, Zell-/Gewebemarker
Beschränkungen Nur für Forschungszwecke einsetzbar

Alternativen

Alternativen zu Antigen "Vimentin (VIM)", Typ "Antikörper" finden
Wirte Kaninchen (116), Maus (106), Huhn (13), Ratte (6), Schaf (6), Ziege (2), Meerschweinchen (2)
Reaktivitäten Human (216), Ratte (Rattus) (94), Maus (85), Huhn (38), Rind (Kuh) (30), Schwein (28), Hund (11), Säugetiere (8), Hamster (7), Ziege (6), Affe (6), Amphibien (4), Potoroo (2), Katze (1), Fisch (1), Pferd (1), Primate (1), Kaninchen (1), Xenopus laevis (1)
Applikationen Western Blot (WB) (161), Immunfluoreszenz (IF) (97), Immunhistochemie (Paraffinschnitte) (IHC (p)) (76), Durchflusszytometrie (FACS) (59), Immunhistochemie (IHC) (58), ELISA (43), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (43), Immunzytochemie (ICC) (26), Immunpräzipitation (IP) (26), Enzyme Immunoassay (EIA) (23), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (4), To be determined by user (TBD) (3), Dot Blot (Dot) (2), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Transfizierte Zellkultur (TCC) (2)
Konjugate FITC (10), Biotin (8), Alexa Fluor 488 (4), HRP (4), PE (4), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
Epitope pSer82 (23), C-Term (2), Center (2), S82 (2), pSer33 (2), pSer38 (2), pSer50 (2), pSer55 (2), pSer6 (2), pSer71 (2), Ab-56 (1), Ab-83 (1), Fragment (1), pSer56 (1), pSer83 (1)