Actinin, alpha (ACTN) Antikörper

Antigen: Actinin, alpha (ACTN)

Klonalität: Monoklonal (SA-20)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343515

Produktbeschreibung

Weitere Bezeichnung: alpha-Actinin

Immunogen: Rabbit skeletal a-actinin. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: SA-20

Beschreibung: Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 3.5µg/ml with the appropriate system to detect a-actinin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 3.5-7µg/ml to detect a-actinin in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Immunohistochemistry(F) At 3.5-7µg/ml to detect a-actinin in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar