Actin, beta (ACTB) Antikörper

Antigen: Actin, beta (ACTB)

Synonyme: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Klonalität: Monoklonal (AC-2)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro))

Produktnummer: ABIN343511

Produktbeschreibung

Weitere Bezeichnung: betalpha-Actin

Immunogen: Slightly modified ß-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-LLe-Ala-Leu-Val-LLe-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. Purification Purified by the goat anti-mouse IgG affinity chromatography.

Format: Affinity-purified

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: AC-2

Beschreibung: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Synonyme: PS1TP5BP1, Actx, beta-actin, E430023M04Rik, ACTB, MGC128179, MGC76228, MGC79692, MGC88999, ps1tp5bp1, LOC442937, LOC563571, A26C1A, A26C1B, ACT-5, act-2, Actb, actb, B-actin, LOC100136013, DKFZp459A196, LOC100174883, LOC100304816, ACTA1

Anwendungen

Applikationshinweise: Western blotting: At 0.5-1g/ml with the appropriate system to detect ß-actin in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.2-0.5µg/ml to detect ß-actin in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.2-0.5µg/ml to detect ß-actin in formalin or acetone fixed tissues. Other applications have not been tested. Optimal dilutions should be determined by end user.

Buffer: Lyophilized from 1.2% sodium acetate, with 2mg BSA and 0.2mg NaN3 as preservative.

Lagerung: At -20°C for one year. After reconstitution, at 4°C for three month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100µg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar