Angiopoietin 2 (ANGPT2) Antikörper

Antigen: Angiopoietin 2 (ANGPT2)

Synonyme: ANG2, AGPT2, Ang2, Agpt2, Ang-2, ang2, ANGPT2, MGC159730, ANGIOPOIETIN-2, ANG-2

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343500

Produktbeschreibung

Weitere Bezeichnung: Angiopoietin-2, ANG2

Immunogen: A synthetic peptide corresponding to a sequence mapping near the C-terminal of human angiopoietin-2, different from the related mouse sequence by two amino acids.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Angiopoietin 1 and Angiopoietin 2 are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie 2. Angiopoietin 2 is only 60% homologous with Angiopoietin 1. Angiopoietin-2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation. Angiopoietin 1 binding to Tie 2 causes phosphorylation of the receptor. Angiopoietin 2 competes for this binding, and thus blocks receptor phosphorylation. Angiopoietin 2 expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2u/ml with the appropriate system to detect ANG2 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 1-2u/ml to detect ANG2 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of rat brain tissue lysis using ANG2 antibody

Immunohistochemical analysis of paraffin-embedded Human placenta sections using ANG2 antibody