Apoptosis Inhibitor 5 (API5) Antikörper

Antigen: Apoptosis Inhibitor 5 (API5)

Synonyme: AAC11, AAC-11, AI196452, api5l1, sr:nyz021, zgc:66211, wu:fb26c03, MGC187857, API-5B, api5-B, MGC53885, api5, API-5A, api5-A, MGC83290, API5, DKFZp459E0724

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN343496

Produktbeschreibung

Weitere Bezeichnung: Apoptosis inhibitor 5, API5

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human API5, identical to the related rat and mouse sequence.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Many growth factors and cytokines act as cellular survival factors by preventing programmed cell death (apoptosis). Apoptosis inhibitor 5 (API5) is an antiapoptotic factor which may have a role in protein assembly. It is a critical determinant of E2F1-induced apoptosis, acting downstream of E2F to suppress E2F-dependent apoptosis without generally blocking E2F-dependent transcription.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 1-2u/ml with the appropriate system to detect API5 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect API5 in formalin fixed and paraffin embedded tissues. Boiling the sections is required. Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis ofRat cardiac muscle tissue lysis using API5 antibody

Immunohistochemical analysis of paraffin-embedded Human epidermic carcinoma sectionsusing API5 antibody