CD22 Molecule (CD22) Antikörper

Antigen: CD22 Molecule (CD22)

Synonyme: SIGLEC2, FLJ22814, SIGLEC-2, MGC130020

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunzytochemie (ICC)

Produktnummer: ABIN343487

Produktbeschreibung

Weitere Bezeichnung: CD22

Immunogen: A peptide mapping at the C-terminal end of human CD22, different from the relative sequence of mouse by three amino acids.

Format: Affinity-purified

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. CD22 is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. 3 CD22 is located within the band region q13.1 of chromosome 19.

Spezifität: Human, mouse, rat. No cross reactivity with other proteins.

Anwendungen

Applikationshinweise: Western blotting: At 0.5-1u/ml with the appropriate system to detect CD22 in cells and tissues. Immunohistochemistry (Paraffin-embedded Sections): At 0.5-1u/ml to detect CD22in formalin fixed and paraffin embedded tissues. Immunohistochemistry(F) At 0.5-1u/ml to detect CD22 in formalin or acetone fixed tissues. Immunocytochemistry Suitable Other applications have not been tested. Optimal dilutions should be determined by end user.

Reinheit: Immunogen affinity purified.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na 2 HPO 4 , 0.05mg Thimerosal, 0.05mg NaN 3 .

Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for longer time. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemical analysis of paraffin-embedded Human appendicitis sectionStaining CD22 in cytoplasmDAB chromogenic reaction